Jeol 2200FS TEM

The Jeol 2200FS is a 200kV FEG transmission cryo-electron microscope with in-column energy filter and a top of the line Gatan K2 Summit director electron detector. It is equipped with a semi-automated data collection system for single-particle samples and tomography.

This machine is primarily used for high-resolution structural biology applications.

Light microscopy

For light microscopy, the RTP has a dual inverted selective plane illumination microscope (diSPIM) from Intelligent Imaging Solutions (3i). This is used for low photobleaching application, high z-resolution and imaging of large samples.

For other light microscopy applications, please contact the SLS Imaging Facility manager Ian at i.j.portman@warwick.ac.uk.

Jeol 2100Plus TEM

The Jeol 2100Plus is a 200kV LaB6 instrument fitted with a Gatan OneView IS camera. It is set up for room temperature or cryo use. This is our workhorse electron microscope and is used for routine work.

Two 3-hour training sessions are usually sufficient to use this machine independently for room-temperature TEM. For cryo-EM, the training process takes a few days.

Cryo-ultramicrotome

Our brand-new cryo-ultramicrotome is set up for use at room temperature or cooled with liquid nitrogen. It can be used for CEMOVIS, Tokuyasu and sectioning of resin-embedded samples.

Electron microscopy sample preparation

We have a dedicated staining bench, cryo-transfer bench, a carbon coater, a glow discharger, and a rapid plunge-freezing device. We can fully support your sample preparation.

Image Analysis

We support analysis of datasets a variety of platforms. ImageJ/FIJI, Imaris, Amira, IMOD. We support RELION, FREALIGN etc for cryoEM structure determination.