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    University of Warwick

    Dr Naila Rabbani

    Naila Rabbani

    TITLE   


    (Previously Naila Ahmed)

    CONTACT   


    Metabolic & Vascular Health
    Warwick Medical School
    CSB, UHCW - Walsgrave
    Clifford Bridge Road
    Coventry
    CV2 2DX
    Tel: 024 7696 8593
    Fax: 024 7696 8653
    Email: N.Rabbani@warwick.ac.uk

     

    RESEARCH PROFILE


    Multi-disciplinary, working in the field of disease mechanisms - particularly in the study of damage to the proteome by glycation, oxidation and nitration, related enzymatic countermeasures and other metabolic dysfunction. Diseases under current investigation are: vascular complications of diabetes, renal failure and ageing ¯ particularly nephropathy and cardiovascular disease. Using systems approach to model dysfunctional lipid metabolism caused by abnormal post-translational lipoprotein modification. Therapeutic problems under current investigation are: high dose thiamine therapy for the prevention of diabetic nephropathy and Nrf2 activators for health benefit. Mechanistic biomarker discovery: for metabolic, vascular and joint health.


    RESEARCH GROUPS


    • Protein Damage and Systems Biology Research Group

    BACKGROUND


    I entered academic life as a mature student in 1995. After graduating in Biological and Medicinal Chemistry, I pursued pre-doctoral research improving analytical methods to identify and quantify markers of protein damage by glycation, oxidation and nitration. Damage to proteins of these types is important in mechanisms of chronic and degenerative disease mediating impairment of structural, catalytic and regulatory proteins. I gained skills and experience in mass spectrometry (LC−MS/MS stable isotopic dilution analysis of amino acids and related glycated, oxidised and nitrated derivatives, and proteomics) mammalian cell culture, pre-clinical animal pharmacology and clinical studies − working with colleagues within the host research team and with several national and international collaborating research groups. I have supervised 4 PhD students, published 76 peer-reviewed articles and 90 conference papers − h-index 31; and I have filed 5 patents. In my post-doctoral research, I continued studies developing LC-MS/MS techniques for comprehensively and quantitatively screening for protein damage in models of disease mechanisms and clinical studies and became world leaders in this field - supported by Welcome Trust. With advanced proteomics techniques, I identified proteins and sites within proteins susceptible to damage by glycation − particularly glycation by the reactive dicarbonyl methylglyoxal (MG): albumin, haemoglobin, lens crystallins and type IV collagen. Markers of damage to albumin and haemoglobin are of diagnostic relevance as markers of glycaemic control and risk of vascular disease development in diabetes. I studied markers of protein damage in clinical and experimental diabetes and diabetic complications, endstage renal disease and dialysis, cirrhosis, Alzheimer?s disease, ageing, arthritis and thermally processed foodstuffs. Damage to lens crystallins and type IV collagen is important mechanistically in the development of cataract and vascular disease, respectively. I gained national and international recognition of my work because of its original, insightful approach. Since April 2007, I have co-directed the Protein Damage and Systems Biology Research Group in Warwick Medical School with Professor Paul J. Thornalley. I specialise in preclinical and clinical studies of protein damage and the anti-stress gene response in disease mechanisms, diagnostics and therapeutics − with spin-off therapeutics development (high dose thiamine therapy for diabetic vascular complications). My current research focus is investigations of damage to lipoproteins and influence of dietary bioactive compounds on lipoprotein synthesis, damage and metabolism - supported by the BHF and BBSRC. My core research project is a study of dicarbonyl glycation of apolipoprotein B100 of low density lipoprotein (LDL) and its importance in dyslipidaemia in diabetes and ageing. This includes use of mathematical models in a systems biology approach to predict consequences of change in lipoprotein function in lipoprotein metabolism following glycation. Improved understanding is also available from studies of high density lipoprotein (HDL) damage in dyslipidaemia and atherosclerosis in diabetes and ageing. I am supervising two post−doctoral research fellows to study physiological damage and functional impairment to HDL − a BHF funded project grant.

    TEACHING PROFILE


    Courses Taught

    • Advances in research methodology and ethics
        Pakistan higher education sponsored workshop at Lahore College Women University
    • The Kidney as an Endocrine Organ

    CURRENT RESEARCH PROJECTS


    • COST Action on Biomimetic Radical Chemistry (CM1201)., Funded by: European Union, Project Start Date: 18/06/2012 Project End Date: 17/06/2016
    • Dicarbonyl stress and dysfunction of the glyoxalase system in periodontal disease, Funded by: Saudi Arabian Ministry of Education, Project Start Date: 01/10/2012 Project End Date: 01/10/2015
    • BIOmarkers of Robustness of Metabolic Homeostasis for Nutrigenomics-derived Health CLAIMS Made on Food (BIOCLAIMS), with Prof Paul J Thornalley, Clinical Sciences Research Institute, Dr Naila Rabbani - Clinical Sciences Research Institute -, Funded by: EU FP7, Project Start Date: 01/03/2010 Project End Date: 28/02/2015
    • Nutrition for Life call via Unilever - FULL, with Prof Paul J Thornalley, Metabolic & Vascular Health, Dr Naila Rabbani - Metabolic & Vascular Health - - - - -, Funded by: Technology Strategy Board, Project Start Date: 01/01/2012 Project End Date: 31/12/2014
    • Effect of glyoxalase 1 silencing and gene deletion on development of diabetic nephropathy, Funded by: Saudi Arabian Ministry of Education, Project Start Date: 01/01/2011 Project End Date: 30/10/2014
    • Molecular mechanisms of glycation and oxidative stress in psychiatric disorders., with Dr Makoto Arai, Funded by: Japan Society for the Promotion of Science, Project Start Date: 01/03/2012 Project End Date: 28/03/2014
    • Anti-stress gene response in cell and tissue ageing: role of transcription factor NF-E2-related factor-2 and effect of dietary activators, Funded by: BBSRC-Unilever, Project Start Date: 01/03/2010 Project End Date: 28/02/2014
    • Glyoxalase Centennial ? 100 years of glyoxalase research and emergence of dicarbonyl stress, Funded by: Biochemical Society, Project Start Date: 27/11/2013 Project End Date: 29/11/2013

      View all Research Projects


    SELECTED PUBLICATIONS


    • Thomas H. Fleming,,Till-Martin Theilen, Jinit Masania, Marius Wunderle, Jamshid Karimi, Spiros Vittas, Rainer Bernauer, Angelika Bierhaus, Naila Rabbani, Paul J. Thornalley, Jens Kroll, Jens Tyedmers, Ralph Nawrotzki, Stephan Herzig, Michael Brownlee and Peter P. Nawroth., (2013) 'Aging-dependent reduction in Glyoxalase-I delays wound healing, Gerontology' in press
    • Ramzi Ajjan,, Toby Gamlen, Kristina Standeven, Salihah Mughal, Katharina Hess, Kerrie Smith, Emma Dunn, Anwar, Muhammad Maqsud, Rabbani, Naila Thornalley, Paul,Helen Philippou and Peter Grant, (2013) 'Diabetes is associated with post-translational modifications in plasminogen resulting in reduced plasmin generation and enzyme specific activity' Blood in press
    • Xue, M.,,Rabbani, N., Momiji, H., Imbasi, P., Anwar, M.M., Kitteringham, N., Parks, B.K., Souma, T., Moriguchi, T., Yamamoto, M. and Thornalley, P.J., (2012) 'Transcriptional control of glyoxalase 1 by Nrf2 provides a stress-responsive defence against dicarbonyl glycation' Biochemical Journal 443 (1), 213 - 222 [article]
    • Larkin, James Robert, Zhang, F., Godfrey, L., Molostvov, Guerman, Zehnder, Daniel, Rabbani, Naila and Thornalley, Paul J.. (2012) 'Glucose-induced down regulation of thiamine transporters in the kidney proximal tubular epithelium produces thiamine insufficiency in diabetes' PLoS One 7 (12), e53175 [article]
    • Lopez-Clavijo, A.F.,,Barrow, M.P., Rabbani, N., Thornalley, P.J. and O?Connor, P.B., (2012) 'Determination of Types and Binding Sites of Advanced Glycation End Products for Substance P.' Analytical Chemistry 84 10568 - 10575

    View all Publications

    Conferences


    RESEARCH DEGREES SUPERVISED

    • Effect of glyoxalase 1 silencing and gene deletion on development of diabetic nephropathy, Date of Completion: 2013
    • Dicarbonyl glycation and protein damage in vascular endothelial cells in hyperglycaemia associated with diabetes., Date of Completion: 2013
    • Protein damage markers in diagnosis, progression and treatment of arthritis, Date of Completion: 2013
    • Mechanism of increased renal clearance of thiamine in hyperglycaemia associated with diabetes., Date of Completion: 2012
    • Glycation in periodontal disease

    Update My Profile on the Warwick eRA Portal
    You can Track My Proposals here
    My Profile last updated: 17/05/2013

    Protein Damage and Systems Biology


    Warwick Systems Biology Centre


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