John Sidda
Register now for the 9th Chemistry and Biology of Natural Products Symposium - Warwick 25-26 June 2015
http://warwick.ac.uk/naturalproducts
Primary Research Objectives:
Creation of mutant Streptomyces strains with targeted gene deletions to overproduce specific secondary metabolites.
Extraction of new natural products from Streptomyces and characterisation by mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy.
Elucidation of the biosynthetic pathway involved in production of a novel series of molecules by genetic manipulation of Streptomyces strains, isotope labelling experiments, heterologous expression of biosynthetic genes and metabolic profiling using liquid chromatography mass spectrometry (LC-MS).
Investigation of the role of transcriptional repressor proteins and low molecular weight signalling molecules in the regulation of natural product biosynthesis in other strains of Streptomyces.
Papers:
2. J. D. Sidda, L. Song, V. Poon, M. Al-Bassam, O. Lazos, M. J. Buttner, G. L. Challis and C. Corre. Discovery of a family of gamma-aminobutyrate ureas via rational derepression of a silent bacterial gene cluster. Chem. Sci. 2014, 5 (1), 86-89. DOI: 10.1039/C3SC52536H
1. J. D. Sidda and C. Corre. Gamma-butyrolactone and furan signalling systems in Streptomyces. Methods in Enzymol. 2012, 517, 71-87. DOI: 10.1016/B978-0-12-404634-4.00004-8
Conference Talks:
'Breaking transcriptional 'locks' for natural product discovery in Streptomyces venezuelae' International Symposium on the Biology of Actinomycetes (ISBA) XVII, Kusadasi, Turkey (2014).
'Discovery and biosynthesis of novel natural products from Streptomyces venezuelae' The Chemistry and Biology of Natural Products Symposium VII, Warwick, UK (2013).
Academic history:
May 2015 - present: IAS Early Career Fellow working in School of Life Sciences/Department of Chemistry, University of Warwick 'Metabolic engineering for natural product discovery in Streptomyces spp.'
2011 - 2015 PhD student in Christophe Corre group: 'Discovery and biosynthesis of novel natural products from Streptomyces venezuelae' - thesis submitted April 2015
2007-2011 University of Warwick: MChem Chemical Biology
4th year project under supervision of Dr. Christophe Corre – ‘Identification and characterisation of novel natural products from Streptomyces’
July-September 2010 Department of Chemistry, University of Warwick
Undergraduate Research Scholarship Scheme (URSS) project under supervision of Prof. Peter Sadler – ‘Square-planar Platinum(II) complex-oligonucleotide interactions’
Other interests:
Travelling
Swimming
Athletics
Wine Appreciation
Contact details:
School of Life Sciences
University of Warwick
Coventry
CV4 7AL
Email j.d.sidda@warwick.ac.uk
Phone +44 (0)2476 573 824
Office C146