Professor David Roper
Professor of Biochemistry and Director of Research
Email: David.Roper@warwick.ac.uk
Phone: 024 765 28369
Office: IBRB 2.25
Twitter: @davidroper2
Roper webpage
Research Clusters
Microbiology & Infectious Disease
Warwick Centres and GRPs
Warwick Antimicrobial Interdisciplinary Centre
Vacancies and Opportunities
For PhD and postdoctoral opportunities, and interest in potential collaborations, please contact me at the above email address.
Research Interests
Research in my group is focused on the way in which pathogenic bacteria make their cell walls grow, how that is related to the ways in which they divide and reproduce and also the relationship of these processes to the discovery and resistance to antibiotics
- Peptidoglycan Biosynthesis: The majority of my work is concentrated on understanding the molecular mechanisms that underpin the biosynthesis of peptidoglycan pathogens with particular emphasis on the way in which cell shape is determined and controlled by RodA-PBP and the wider elongasome. This involved coordination of processes from inside to outside the cell membrane
- Bacteria Cell division: Cell division is the largest morphological change that any cell undergoes and has common elements in all forms of life. In bacteria this is controlled by a dynamic complex of proteins, many of which are located at or in the cell membrane making their study and analysis challenging. FtsZ and the proteins which interact are of key interest in that respect.
- New antibacterial drug discovery: Our studies on cell division and their linkage to peptidoglycan synthesis has an intimate connection to the targets of existing and potentially future antimicrobial drugs. We are investigating a number of these including the potential of the lipid II polymerisation process as a target for such drugs.
- Molecular Mechanisms of Antibiotic resistance. We are interested in the molecular mechanisms of antibiotic resistance, in relation to the biosynthesis of the cell wall including the mode of action of the lipid II sequestering antibiotic, vancomycin and our discovery of the way in which D-cycloserine targets three different aspects of peptidoglycan biosynthesis.
Research: Technical Summary
David Roper has a background in microbial biochemistry and was awarded an MRC Career Development Award fellowship at the University of York prior to taking an academic position at the University of Warwick, where he has now Professor of Biochemistry and Director of Research.
The Roper group uses interdisciplinary approaches from basic microbiology to molecular structure determination by X-ray crystallography, relating enzyme and protein mechanism to structure and function at a cellular level. His research encompasses both fundamental and translation approaches including assay development and drug discovery approaches. In addition, his research group uses a number of approaches to reengineer pathway intermediates as chemical probes, substrates and inhibitors to allows novel insight to the biology underpinning these pathways which may lead to biotechnological exploration and exploitation. This includes the design and synthesis of chemical mimetic small molecule probes for mechanistic and translational studies.
- 2018 - . Professor, School of Life Sciences, University of Warwick
- 2020 - 2021. Vagelos College of Physicians & Surgeons, Columbia University New York, 2020 visiting Schaefer Research Scholar
- 2018 - 2019. Academy of Medical Sciences, Hamied Foundation UK-India AMR Visiting Professorships
- 2014 - 2017. Reader in Structural Biology, School of Life Sciences, University of Warwick
- 2015 - 2016. BBSRC-RSE Enterprise Fellow, Antimicrobial Discovery Solutions Ltd.
- 2006 - 2014. Associate Professor of Structural Biology, Biological Sciences, University of Warwick.
- 2001 - 2006. Lecturer in Structural Biology, Biological Sciences, University of Warwick
- 1996 - 2001. MRC Career Development Fellow, Dept Chemistry, University of York.
- 1990 - 1996. SERC & BBSRC Post-doctoral Fellow, Dept Chemistry, University of York.
- 1987-1990. Ph.D. Biochemistry, Dept Biochemistry, University of Leicester.
- 1983-1997. B.Sc. Biochemistry, Dept Biochemistry, University College Cardiff.
- Nygaard, Rie, Graham, Chris L. B., Belcher Dufrisne, Meagan, Colburn, Jonathan D., Pepe, Joseph, Hydorn, Molly A., Corradi, Silvia, Brown, Chelsea M., Ashraf, Khuram U., Vickery, Owen N., Briggs, Nicholas, Deering, John, Kloss, Brian, Botta, Bruno, Clarke, Oliver B., Columbus, Linda, Dworkin, Jonathan, Stansfeld, Phillip J., Roper, David I., Mancia, Filippo, 2023. Structural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex. Nature Communications, 14 (1)
- Graham, Chris L. B., Bryant, Jack, Roper, David I., Banzhaf, Manuel, 2023. Membrane staining and phospholipid tracking in Pseudomonas aeruginosa PAO1 using the phosphatidylcholine mimic propargyl-choline. Access Microbiology
- 'J?nior, Jos? Renato Pattaro, 'Caruso, ?caro Putinhon, 'de S?, J?ssica Mar?stica, 'Mezalira, Taniara Suelen, 'de Souza Lima, Diego, 'Pilau, Eduardo Jorge, 'Roper, David I., 'Fernandez, Maria Aparecida, 'Vicente Seixas, Flavio Augusto, 2022. 'Characterization of secondary structure and thermal stability by biophysical methods of the D-alanyl,D-alanine ligase B protein from Escherichia coli. Protein & Peptide Letters, 29 (5), pp. 448-459
- 'Dhaonoa, Gurneet K., 'Kushnir, Inbar, 'Qimron, Udi, 'Roper, David I., 'Sagona, Antonia P., 2022. 'Investigating the effect of bacteriophages on bacterial FtsZ localisation. Frontiers in Cellular and Infection Microbiology-Microbiome in Health and Disease, 12
- Ashraf, Khuram U., Nygaard, Rie, Vickery, Owen N., Erramilli, Satchal K., Herrera, Carmen M., McConville, Thomas H., Petrou, Vasileios I., Giacometti, Sabrina I., Belcher Dufrisne, Meagan, Nosol, Kamil, Zinkle, Allen P., Graham, Chris L. B., Loukeris, Michael, Kloss, Brian, Skorupinska-Tudek, Karolina, Swiezewska, Ewa, Roper, David I., Clarke, Oliver B., Uhlemann, Anne-Catrin, Kossiakof, Anthony A. et al (Select to open full list), 2022. Structural basis of lipopolysaccharide maturation by the WaaL O-antigen ligase. Nature, 604, pp. 371-376
- Dowson, Amanda J., Lloyd, Adrian J., Cuming, Andrew C., Roper, David I., Frigerio, Lorenzo, Dowson, Christopher G., 2022. Plant peptidoglycan precursor biosynthesis : conservation between moss chloroplasts and Gram-negative bacteria. Plant Physiology, 190 (1), pp. 165-179
- Tran, Wendy, Kusay, Ali S., Hawkins, Paige M. E., Cheung, Chen-Yi, Nagalingam, Gayathri, Pujari, Venugopal, Ford, Daniel J., Stoye, Alexander, Ochoa, Jessica L., Audette, Rebecca E., Hortle, Elinor, Oehlers, Stefan H., Charman, Susan A., Linington, Roger G., Rubin, Eric J., Dowson, Christopher G., Roper, David I., Crick, Dean C., Balle, Thomas, Cook, Gregory M. et al (Select to open full list), 2021. Synthetic sansanmycin analogues as potent mycobacterium tuberculosis translocase I inhibitors. Journal of Medicinal Chemistry, 64 (23), pp. 17326-17345
- Briggs, Nicholas, Bruce, Kevin E., Naskar, Souvik, Winkler, Malcolm E., Roper, David I., 2021. The pneumococcal divisome : dynamic control of streptococcus pneumoniae cell division. Frontiers in Microbiology, 12
- Graham, Chris L. B., Newman, Hector, Gillett, Francesca, Smart, Katie, Briggs, Nicholas, Banzhaf, Manuel, Roper, David I., 2021. A dynamic network of proteins facilitate cell envelope biogenesis in gram-negative bacteria. International Journal of Molecular Sciences, 22 (23)
- Aggarwal, Surya D., Lloyd, Adrian J., Yerneni, Saigopalakrishna S., Narciso, Ana Rita, Shepherd, Jennifer, Roper, David I., Dowson, Christopher G., Filipe, Sergio R, Hiller, N Luisa, 2021. A molecular link between cell wall biosynthesis, translation fidelity, and stringent response in Streptococcus pneumoniae. Proceedings of the National Academy of Sciences of the United States of America, 118 (14)
- York, Anna, Lloyd, Adrian J., del Genio, Charo I., Shearer, Jonathan, Hinxman, Karen, Fritz, Konstantin, Fülöp, Vilmos, Dowson, Christopher G., Khalid, Syma, Roper, David I., 2021. Structure-based modeling and dynamics of MurM, a Streptococcus pneumoniae penicillin resistance determinant present at the cytoplasmic membrane. Structure, 29 (7), pp. 731-742
- Cook, Jonathan P., Baverstock, Tyler, McAndrew, Martin B. L., Stansfeld, Phillip J., Roper, David I., Crow, Allister, 2020. Insights into intrinsic resistance and bacterial cell division from a structure of EnvC bound to the FtsX periplasmic domain. Proceedings of the National Academy of Sciences of the United States of America, 117 (45), pp. 28355-28365
- Bryant, Jack Alfred, Morris, Faye C., Knowles, Timothy J., Maderbocus, Riyaz, Heinz, Eva, Boelter, Gabriela, Alodaini, Dema, Colyer, Adam, Wotherspoon, Peter J., Staunton, Kara A., Jeeves, Mark, Browning, Douglas F., Sevastsyanovich, Yanina R., Wells, Timothy J., Rossiter, Amanda E., Bavro, Vassiliy N., Sridhar, Pooja, Ward, Douglas G., Chong, Zhi-Soon, Goodall, Emily CA et al (Select to open full list), 2020. Structure of dual BON-domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation. eLife, 9
- Catherwood, Anita C., Lloyd, Adrian J., Tod, Julie A., Chauhan, Smita, Slade, Susan E., Walkowiak, Grzegorz P., Galley, Nicola F., Punekar, Avinash S., Smart, Katie, Rea, Dean, Evans, Neil D., Chappell, Michael J., Roper, David I., Dowson, Christopher G., 2020. Substrate and stereochemical control of peptidoglycan cross-linking by transpeptidation by Escherichia coli PBP1B. Journal of the American Chemical Society, 142 (11), pp. 5034-5048
- 'Lockey, Christine, 'Edwards, Richard J., 'Roper, David I., 'Dixon, Ann M., 2020. 'The extracellular domain of two-component system sensor kinase VanS from streptomyces coelicolor binds Vancomycin at a newly identified binding site. Scientific Reports, 10 (1)
- Kuru, Erkin, Radkov, Atanas, Meng, Xin, Egan, Alexander, Alvarez, Laura, Dowson, Amanda, Booher, Garrett, Breukink, Eefjan, Roper, David I., Cava, Felipe, Vollmer, Waldemar, Brun, Yves, VanNieuwenhze, Michael S., 2019. Mechanisms of incorporation for D-amino acid probes that target peptidoglycan biosynthesis. ACS Chemical Biology, 14 (12), pp. 2745-2756
- Teo, Alvin C. K., Lee, Sarah C., Pollock, Naomi L., Stroud, Zoe, Hall, Stephen, Thakker, Alpesh, Pitt, Andrew R., Dafforn, Timothy R., Spickett, Corinne M., Roper, David I., 2019. Analysis of SMALP co-extracted phospholipids shows distinct membrane environments for three classes of bacterial membrane protein. Scientific Reports, 9 (1)
- Pollock, Naomi, Rai, Megha, Simon, Kailene S., Hesketh, Sophie J., Teo, Alvin C. K., Parmar, Mayuriben, Sridhar, Pooja, Collins, Richard, Lee, Sarah C., Stroud, Zoe N., Bakker, Saskia, Muench, Stephen P., Barton, C. Howard, Hurlbut, Gregory, Roper, David I., Smith, Corinne J., Knowles, Timothy J., Spickett, Corinne M., East, J. Malcolm, Postis, Vincent et al (Select to open full list), 2019. SMA-PAGE : a new method to examine complexes of membrane proteins using SMALP nano-encapsulation and native gel electrophoresis. Biochimica et Biophysica Acta (BBA) - Biomembranes, 186 (8), pp. 1437-1445
- Massarweh, Ahmad, Bosco, Michael, Chantret, Isabelle, Léger, Thibaut, Jamal, Layla, Roper, David I., Dowson, Christopher G., Busca, Patricia, Bouhss, Ahmed, Gravier-Pelletier, Christine, Moore, Stuart E. H., 2019. Bacterial lipid II analogs : novel in vitro substrates for mammalian oligosaccharyl diphosphodolichol diphosphatase (DLODP) activities. Molecules, 24 (11)
- Lee, Sarah C., Collins, Richard, Lin, Yu-pin, Jamshad, Mohammed, Broughton, Claire E., Harris, Sarah A., Hanson, Benjamin S, Tognoloni, Cecilia, Parslow, Rosemary A., Terry, Ann E., Rodger, Alison, Smith, Corinne J., Edler, Karen J., Ford, Robert, Roper, David I., Dafforn, Timothy R., 2019. Nano-encapsulated escherichia coli divisome anchor ZipA, and in complex with FtsZ. Scientific Reports, 9 (1)
- Cain, Ricky, Salimraj, Ramya, Punekar, Avinash S., Bellini, Dom, Fishwick, Colin W. G., Czaplewski, Lloyd, Scott, David J., Harris, Gemma, Dowson, Christopher G., Lloyd, Adrian J., Roper, David I., 2019. Structure-guided enhancement of selectivity of chemical probe inhibitors targeting bacterial seryl-tRNA synthetase. Journal of Medicinal Chemistry, 62 (21), pp. 9703-9717
- Simpson, Daniel H., Hapeshi, Alexia, Rogers, Nicola J., Brabec, Viktor, Clarkson, Guy J., Fox, David J., Hrabina, Ondrej, Kay, Gemma L., King, Andrew, Malina, Jaroslav, Millard, Andrew D., Moat, John, Roper, David I., Song, Hualong, Waterfield, Nicholas R., Scott, Peter, 2019. Metallohelices that kill Gram-negative pathogens using intracellular antimicrobial peptide pathways. Chemical Science, 10, pp. 9708-9720
- Hrast, Martina, Jukic, Marko, Patin, Delphine, Tod, Julie, Dowson, Christopher G., Roper, David I., Barreteau, Hélène, Gobec, Stanislav, 2018. In silico identification, synthesis and biological evaluation of novel tetrazole inhibitors of MurB. Chemical Biology & Drug Design, 91 (6), pp. 1101-1112
- Punekar, Avinash S., Samsudin, Firdaus, Lloyd, Adrian J., Dowson, Christopher G., Scott, David J., Khalid, Syma, Roper, David I., 2018. The role of the jaw subdomain of peptidoglycan glycosyltransferases for lipid II polymerization. The Cell Surface, 2, pp. 54-66
- Ábrányi-Balogh, Péter, Petri, László, Imre, Tímea, Szijj, Péter, Scarpino, Andrea, Hrast, Martina, Mitrovic, Ana, Fonovic, Ur?a Pecar, Németh, Krisztina, Barreteau, Hélène, Roper, David I., Horváti, Kata, Ferenczy, György G, Kos, Janko, Ila?, Janez, Gobec, Stanislav, Keseru, György M, 2018. A road map for prioritizing warheads for cysteine targeting covalent inhibitors. European Journal of Medicinal Chemistry, 160, pp. 94-107
- Ábrányi-Balogh, Péter, Petri, László, Imre, Tímea, Szijj, Péter, Scarpino, Andrea, Hrast, Martina, Mitrovic, Ana, Fonovic, Ur?a Pecar, Németh, Krisztina, Barreteau, Hélène, Roper, David I., Horváti, Kata, Ferenczy, György G., Kos, Janko, Ila?, Janez, Gobec, Stanislav, Keseru, György M., 2018. A road map for prioritizing warheads for cysteine targeting covalent inhibitors. European Journal of Medicinal Chemistry, 160, pp. 94-107
- Meyer, Karen, Addy, Christine, Akashi, Satoko, Roper, David I., Tame, Jeremy R. H., 2018. The crystal structure and oligomeric form of Escherichia coli L,D-carboxypeptidase A. Biochemical and Biophysical Research Communications, 499 (3), pp. 594-599
- Ábrányi-Balogh, Péter, Petri, László, Imre, Tímea, Szijj, Péter, Scarpino, Andrea, Hrast, Martina, Mitrovic, Ana, Fonovic, Ur?a Pecar, Németh, Krisztina, Barreteau, Hélène, Roper, David I., Horváti, Kata, Ferenczy, György G, Kos, Janko, Ila?, Janez, Gobec, Stanislav, Keseru, György M, 2018. A road map for prioritizing warheads for cysteine targeting covalent inhibitors.. European Journal of Medicinal Chemistry, 160, pp. 94-107
- Vajs, Jure, Proud, Conor, Brozovic, Anamaria, Gazvoda, Martin, Lloyd, Adrian J., Roper, David I., Osmak, Maja, Ko?mrlj, Janez, Dowson, Christopher G., 2017. Diaryltriazenes as antibacterial agents against methicillin resistant Staphylococcus aureus (MRSA) and Mycobacterium smegmatis. European Journal of Medicinal Chemistry, 127, pp. 223-234
- Sychantha, David, Jones, Carys S., Little, Dustin J., Moynihan, Patrick J., Robinson, Howard, Galley, Nicola F., Roper, David I., Dowson, Christopher G., Howell, P. Lynne, Clarke, Anthony J., 2017. In vitro characterization of the antivirulence target of Gram-positive pathogens, peptidoglycan O-acetyltransferase A (OatA). PLOS Pathogens, 13 (10)
- Batson, Sarah, de Chiara, Cesira, Majce, Vita, Lloyd, Adrian J., Gobec, Stanislav, Rea, Dean, Fülöp, Vilmos, Thoroughgood, Christopher W., Simmons, Katie J., Dowson, Christopher G., Fishwick, Colin W. G., de Carvalho, Luiz Pedro S., Roper, David I., 2017. Inhibition of D-Ala : D-Ala ligase through a phosphorylated form of the antibiotic D-cycloserine. Nature Communications, 8 (1)
- Tran, Anh T., Watson, Emma E., Pujari, Venugopal, Conroy, Trent, Dowman, Luke J., Giltrap, Andrew M., Pang, Angel, Wong, Weng Ruh, Linington, Roger G., Mahapatra, Sebabrata, Saunders, Jessica, Charman, Susan A., West, Nicholas P., Bugg, Tim, Tod, Julie, Dowson, Christopher G., Roper, David I., Crick, Dean C., Britton, Warwick J., Payne, Richard J., 2017. Sansanmycin natural product analogues as potent and selective anti-mycobacterials that inhibit lipid I biosynthesis. Nature Communications, 8
- Calvez, Philippe, Breukink, Eefjan, Roper, David I., Dib, Mélanie, Contreras-Martel, Carlos, Zapun, André, 2017. Substitutions in PBP2b from ?-lactam resistant Streptococcus pneumoniae have different effects on enzymatic activity and drug reactivity. Journal of Biological Chemistry, 292 (7), pp. 2854-2865
- Tran, Anh T., Watson, Emma E., Pujari, Venugopal, Conroy, Trent, Dowman, Luke J., Giltrap, Andrew M., Pang, Angel, Wong, Weng-Ruh, Linington, Roger G., Saunders, Jessica, Charman, Susan A., West, Nicholas P., Bugg, Tim, Tod, Julie, Dowson, Christopher G., Roper, David I., Crick, Dean C., Britton, Warwick J., Payne, Richard J., 2017. Sansanmycin natural product analogues as potent and selective anti-mycobacterials that inhibit lipid I biosynthesis.. Nature Communications, 8
- Kaner, Rebecca A., Allison, Simon A., Faulkner, Alan D., Simpson, Daniel H., Waterfield, Nicholas R., Phillips, Roger M., Roper, David I., Shepherd, Samantha L., Scott, Peter, 2016. Anticancer metallohelices : nanomolar potency and high selectivity. Chemical Science, 7 (2), pp. 951-958
- Usha, Veeraraghavan, Lloyd, Adrian J., Roper, David I., Dowson, Christopher G., Kozlov, Guennadi, Gehring, Kalle, Chauhan, Smita, Imam, Hasan T., Blindauer, Claudia A., Besra, Gurdyal S., 2016. Reconstruction of diaminopimelic acid biosynthesis allows characterisation of Mycobacterium tuberculosis N-succinyl-L,L-diaminopimelic acid desuccinylase. Scientific Reports, 6
- Broughton, Claire E., Berg, Hugo van den, Wemyss, Alan M., Roper, David I., Rodger, Alison, 2016. Beyond the discovery void : new targets for antibacterial compounds. Science Progress, 99 (2), pp. 153-182
- Dow, Claire E., Berg, Hugo van den, Roper, David I., Rodger, Alison, 2015. Biological insights from a simulation model of the critical FtsZ accumulation required for prokaryotic cell division. Biochemistry, 54 (24), pp. 3803-3813
- Broughton, Claire E., Roper, David I., Berg, Hugo van den, Rodger, Alison, 2015. Bacterial cell division : experimental and theoretical approaches to the divisome. Science Progress, 98 (4), pp. 313-345
- Zuegg, Johannes, Muldoon, Craig, Adamson, George, McKeveney, Declan, Thanh, Giang Le, Premraj, Rajaratnam, Becker, Bernd, Cheng, Mu, Elliott, Alysha G., Huang, Johnny X., Butler, Mark S., Bajaj, Megha, Seifert, Joachim, Singh, Latika, Galley, Nicola F., Roper, David I., Lloyd, Adrian J., Dowson, Christopher G., Cheng, Ting-Jen, Cheng, Wei-Chieh et al (Select to open full list), 2015. Carbohydrate scaffolds as glycosyltransferase inhibitors with in vivo antibacterial activity. Nature Communications, 6, pp. 1-11
- Li, Qiong, Cheng, Wang, Morlot, Cécile, Bai, Xiao-Hui, Jiang, Yong-Liang, Wang, Wenjia, Roper, David I., Vernet, Thierry, Dong, Yu-Hui, Chen, Yuxing, Zhou, Cong-Zhao, 2015. Full-length structure of the major autolysin LytA. Acta Crystallographica Section D Biological Crystallography, 71 (6), pp. 1373-1381
- Teo, Alvin, Roper, David I., 2015. Core steps of membrane-bound peptidoglycan biosynthesis : recent advances, insight and opportunities. Antibiotics, 4 (4), pp. 495-520
- Rodolis, Maria T., Mihalyi, Agnes, O'Reilly, Amy M., Slikas, Justinas, Roper, David I., Hancock, Robert E. W., Bugg, Tim, 2014. Identification of a novel inhibition site in Translocase MraY based upon the site of interaction with Lysis Protein E from Bacteriophage ?X174. Chembiochem, 15 (9), pp. 1300-1308
- Galley, Nicola F., O'Reilly, Amy M., Roper, David I., 2014. Prospects for novel inhibitors of peptidoglycan transglycosylase. Bioorganic Chemistry, 55 (100), pp. 16-26
- Braddick, Darren, Sandhu, Sandeep, Roper, David I., Chappell, M. J. (Michael J.), Bugg, Tim, 2014. Observation of the time-course for peptidoglycan lipid intermediate II polymerization by Staphylococcus aureus monofunctional transglycosylase. Microbiology, 160 (Part 8), pp. 1628-1636
- Faulkner, Alan D., Kaner, Rebecca A., Abdallah, Qasem M. A., Clarkson, Guy J., Fox, David J., Gurnani, Pratik, Howson, Suzanne E., Phillips, Roger M., Roper, David I., Simpson, Daniel H., Scott, Peter, 2014. Asymmetric triplex metallohelices with high and selective activity against cancer cells. Nature Chemistry, 6 (9), pp. 797-803
- Paulin, Sarah, Jamshad, Mohammed, Dafforn, Tim, Garcia-Lara, Jorge, Foster, Simon J., Galley, Nicola F., Roper, David I., Rosado, Helena, Taylor, Peter W., 2014. Surfactant-free purification of membrane protein complexes from bacteria : application to the staphylococcal penicillin-binding protein complex PBP2/PBP2a. Nanotechnology, 25 (28)
- Meigh, Louise, Greenhalgh, Sophie A., Rodgers, Thomas L., Cann, Martin J., Roper, David I., Dale, Nicholas, 2013. CO2 directly modulates connexin 26 by formation of carbamate bridges between subunits. eLife, 2
- Lloyd, Adrian J., Potter, Nicola J., Fishwick, Colin W. G., Roper, David I., Dowson, Christopher G., 2013. Adenosine tetraphosphoadenosine drives a continuous ATP-release assay for aminoacyl-tRNA synthetases and other adenylate-forming enzymes. ACS Chemical Biology, 8 (10), pp. 2157-2163
- Pacheco-Gómez, Raúl, Cheng, Xi, Hicks, Matthew R., Smith, Corinne J., Roper, David I., Addinall, Stephen G., Rodger, Alison, Dafforn, Tim, 2013. Tetramerization of ZapA is required for FtsZ bundling. Biochemical Journal, 449 (3), pp. 795-802
- Dow, Claire E., Rodger, Alison, Roper, David I., Berg, Hugo van den, 2013. A model of membrane contraction predicting initiation and completion of bacterial cell division. Integrative Biology, pp. 778-795
- Lloyd, Adrian J., Potter, Nicola J., Fishwick, Colin W. G., Roper, David I., Dowson, Christopher G., 2013. Adenosine Tetraphosphoadenosine drives a continuous ATP-release assay for Aminoacyl-tRNA synthetases and other Adenylate-forming enzymes. ACS Chemical Biology, 8 (10), pp. 2157-2163
- Majce, Vita, Ruane, Karen M., Gobec, Stanislav, Roper, David I., 2013. Crystallization and preliminary X-ray analysis of a UDP-MurNAc-tripeptideD-alanyl-D-alanine-adding enzyme (PaMurF) from Pseudomonas aeruginosa. Acta Crystallographica. Section F: Structural Biology and Crystallization Communications, 69 (number 5), pp. 503-505
- Zapun, André, Philippe, Jules, Abrahams, Katherine A., Signor, Luca, Roper, David I., Breukink, Eefjan, Vernet, Thierry, 2013. In vitro reconstitution of peptidoglycan assembly from the gram-positive pathogen streptococcus pneumoniae. ACS Chemical Biology, 8 (12), pp. 2688-2696
- Valegård, Karin, Iqbal, Aman, Kershaw, Nadia J., Ivison, David, Généreux, Catherine, Dubus, Alain, Blikstad, Cecilia, Demetriades, Marina, Hopkinson, Richard J., Lloyd, Adrian J., Roper, David I., Schofield, Christopher J., Andersson, Inger, McDonough, Michael A., 2013. Structural and mechanistic studies of theorf12 gene product from the clavulanic acid biosynthesis pathway. Acta Crystallographica Section D Biological Crystallography, 69 (8), pp. 1567-1579
- Ruane, Karen M., Lloyd, Adrian J., Fülöp, Vilmos, Dowson, Christopher G., Barreteau, Hélène, Boniface, Audrey, Dementin, Sébastien, Blanot, Didier, Mengin-Lecreulx, Dominique, Gobec, Stanislav, Dessen, Andréa, Roper, David I., 2013. Specificity determinants for lysine incorporation in staphylococcus aureus peptidoglycan as revealed by the structure of a MurE enzyme ternary complex. Journal of Biological Chemistry, 288 (46)
- Turner, Daniel J., Portman, Ian, Dafforn, Tim, Rodger, Alison, Roper, David I., Smith, Corinne J., Turner, Matthew S., 2012. The Mechanics of FtsZ fibers. Biophysical Journal, Vol.102 (No.4), pp. 731-738
- Yoshida, Hisashi, Kawai, Fumihiro, Obayashi, Eiji, Akashi, Satoko, Roper, David I., Tame, Jeremy R.H., Park, Sam-Yong, 2012. Crystal structures of penicillin-binding protein 3 (PBP3) from methicillin-resistant staphylococcus aureus in the apo and cefotaxime-bound forms. Journal of Molecular Biology, 423 (3), pp. 351-364
- Hattersley, John G., Pérez-Velázquez, J., Chappell, M. J. (Michael J.), Bearup, Daniel James, Roper, David I., Dowson, Christopher G., Bugg, Tim, Evans, Neil D., 2011. Indistinguishability and identifiability of kinetic models for the MurC reaction in peptidoglycan biosynthesis. Computer Methods and Programs in Biomedicine, Vol.104 (No.2), pp. 70-80
- Pacheco-Gómez, Raúl, Roper, David I., Dafforn, Tim, Rodger, Alison, 2011. The pH dependence of polymerization and bundling by the essential bacterial cytoskeltal protein FtsZ. PLoS One, Vol.6 (No.6)
- Bugg, Tim, Braddick, Darren, Dowson, Christopher G., Roper, David I., 2011. Bacterial cell wall assembly : still an attractive antibacterial target. Trends in Biotechnology, Vol.29 (No.4), pp. 167-173
- Kawai, Fumihiro, Clarke, Thomas B., Roper, David I., Han, Gab-Jo, Hwang, Kwang Yeon, Unzai, Satoru, Obayashi, Eiji, Park, Sam-Yong, Tame, Jeremy R. H., 2010. Crystal structures of penicillin-binding proteins 4 and 5 from Haemophilus influenzae. Journal of Molecular Biology, Vol.396 (No.3), pp. 634-645
- Batson, Sarah, Rea, Dean, Fülöp, Vilmos, Roper, David I., 2010. Crystallization and preliminary X-ray analysis of a D-alanyl-D-alanine ligase (EcDdlB) from Escherichia coli. Journal of Experimental Psychology: Human Perception and Performance, Vol.66 (No.4), pp. 405-408
- Clarke, Thomas B., Kawai, Fumihiro, Park, Sam-Yong, Tame, Jeremy R. H., Dowson, Christopher G., Roper, David I., 2009. Mutational analysis of the substrate specificity of Escherichia coli penicillin binding protein 4. Biochemistry, Vol.48 (No.12), pp. 2675-2683
- Sova, Matej, Cadez, Gasper, Turk, Samo, Majce, Vita, Polanc, Slovenko, Batson, Sarah, Lloyd, Adrian, Roper, David I., Fishwick, Colin W. G., Gobec, Stanislav, 2009. Design and synthesis of new hydroxyethylamines as inhibitors of D-alanyl-D-lactate ligase (VanA) and D-alanyl-D-alanine ligase (DdlB). Bioorganic & Medicinal Chemistry Letters, Vol.19 (No.5), pp. 1376-1379
- Cressina, Elena, Lloyd, Adrian J., De Pascale, Gianfranco, Mok, B. James, Caddick, Stephen, Roper, David I., Dowson, Christopher G., Bugg, Tim, 2009. Inhibition of tRNA-dependent ligase MurM from Streptococcus pneumoniae by phosphonate and sulfonamide inhibitors. Bioorganic & Medicinal Chemistry, Vol.17 (No.9), pp. 3443-3455
- Paradis-Bleau, Catherine, Lloyd, Adrian, Sanschagrin, Francois, Maaroufi, Halim, Clarke, Thomas B., Blewett, Ann, Dowson, Christopher G., Roper, David I., Bugg, Tim, Levesque, Roger C., 2009. Pseudomonas aeruginosa MurE amide ligase : enzyme kinetics and peptide inhibitor. Biochemical Journal, Vol.421 (No. 2), pp. 263-272
- Usha, Veeraraghavan, Dover, Lynn G., Roper, David I., Fuetterer, Klaus, Besra, Gurdyal S., 2009. Structure of the diaminopimelate epimerase DapF from Mycobacterium tuberculosis. Acta Crystallographica Section D: Biological Crystallography, Vol.65 (No.4), pp. 383-387
- Lloyd, Adrian J., Gilbey, Andrea M., Blewett, Anne M., De Pascale, Gianfranco, El Zoeiby, Ahmed, Levesque, Roger C., Catherwood, Anita C., Tomasz, Alexander, Bugg, Tim, Roper, David I., Dowson, Christopher G., 2008. Characterization of tRNA-dependent peptide bond formation by MurM in the synthesis of Streptococcus pneumoniae peptidoglycan. Journal of Biological Chemistry, Vol.283 (No.10), pp. 6402-6417
- Usha, Veeraraghavan, Dover, Lynn G., Roper, David I., Besra, Gurdyal S., 2008. Characterization of Mycobacterium tuberculosis diaminopimelic acid epimerase: paired cysteine residues are crucial for racemization. FEMS Microbiology Letters, Vol.280 (No.1), pp. 57-63
- Rea, Dean, Hovington, Rebecca, Rakus, John F., Gerlt, John A., Fülöp, Vilmos, Bugg, Tim, Roper, David I., 2008. Crystal structure and functional assignment of YfaU, a metal ion dependent class II aldolase from Escherichia coli K12. Biochemistry, Vol.47 (No.38), pp. 9955-9965
- De Pascale, Gianfranco, Lloyd, Adrian J., Schouten, James A., Gilbey, Andrea M., Roper, David I., Dowson, Christopher G., Bugg, Tim, 2008. Kinetic characterization of lipid II-Ala:Alanyl-tRNA ligase (MurN) from streptococcus pneumoniae using semisynthetic aminoacyl-lipid II substrates. Journal of Biological Chemistry, Vol.283 (No.50), pp. 34571-34579
- Alderwick, Luke J., Dover, Lynn G., Veerapen, Natacha, Gurcha, Sudagar S., Kremer, Laurent, Roper, David I., Pathak, Ashish K., Reynolds, Robert C., Besra, Gurdyal S., 2008. Expression, purification and characterisation of soluble GlfT and the identification of a novel galactofuranosyltransferase Rv3782 involved in priming GlfT-mediated galactan polymerisation in Mycobacterium tuberculosis. Protein Expression and Purification, Vol.58 (No.2), pp. 332-341
- Izumi, Atsushi, Rea, Dean, Adachi, Tomoko, Unzai, Satoru, Park, Sam-Yong, Roper, David I., Tame, Jeremy R. H., 2007. Structure and mechanism of HpcG, a hydratase in the homoprotocatechuate degradation pathway of Escherichia coli. Journal of Molecular Biology, Vol.370 (No.5), pp. 899-911
- Rea, Dean, Fülöp, Vilmos, Bugg, Tim, Roper, David I., 2007. Structure and mechanism of HpcH : a metal ion dependent class II aldolase from the homoprotocatechuate degradation pathway of Escherichia coli. Journal of Molecular Biology, Vol.373 (No.4), pp. 866-876
- Lee, Sarah C., Stoilova-Mcphie, Svetla, Baxter, Laura, Fülöp, Vilmos, Henderson, Janey, Rodger, Alison, Roper, David I., Scott, David J., Smith, Corinne J., Morgan, J. Alun W., 2007. Structural characterisation of the insecticidal toxin XptA1, reveals a 1.15 MDa tetramer with a cage-like structure. Journal of Molecular Biology, Vol.366 (No.5), pp. 1558-1568
- Lysenko, Elena S., Clarke, Thomas B., Shchepetov, Mikhail, Ratner, Adam J., Roper, David I., Dowson, Christopher G., Weiser, Jeffrey N., 2007. Nod1 signaling overcomes resistance of S. pneumoniae to opsonophagocytic killing. PLoS Pathogens, Vol.3 (No.8), pp. 1073-1081
- Cressina, Elena, Lloyd, Adrian J., De Pascale, Gianfranco, Roper, David I., Dowson, Christopher G., Bugg, Tim, 2007. Adenosine phosphonate inhibitors of lipid II: Alanyl tRNA ligase MurM from Streptococcus pneumoniae. Bioorganic & Medicinal Chemistry Letters, Vol.17 (No.16), pp. 4654-4656
- Lysenko, Elena S., Clarke, Thomas B., Shchepetov, Mikhail, Ratner, Adam J., Roper, David I., Dowson, Christopher G., Weiser, Jeffrey N., 2007. Nod1 signaling overcomes resistance of S. pneumoniae to opsonophagocytic killing. PL o S Pathogens, Vol.3 (No.8), pp. 1073-1081
- Tötemeyer, Sabine, Sheppard, Mark, Lloyd, Adrian, Roper, David I., Dowson, Christopher G., Underhill, David, Murray, Peter, Maskell, Duncan, Bryant, Clare, 2006. IFN-gamma enhances production of nitric oxide from macrophages via a mechanism that depends on nucleotide oligomerization domain-2. Journal of Immunology, Vol.176 (No.8), pp. 4804-4810
- Adachi, Tomoko, Izumi, Atsushi, Rea, Dean, Park, Sam-Yong, Tame, Jeremy R. H., Roper, David I., 2006. Expression, purification and crystallization of 2-oxo-hept-4-ene-1,7-dioate hydratase (HpcG) from Escherichia coli C. Acta Crystallographica Section F-Structural Biology And Crystallization Communicati, 62 (Part 10), pp. 1010-1012
- Usha, Veeraraghavan, Dover, Lynn G., Roper, David I., Lloyd, Adrian J., Besra, Gurdyal S., 2006. Use of a codon alteration strategy in a novel approach to cloning the Mycobacterium tuberculosis diaminopimelic acid epimerase. FEMS Microbiology Letters, 262 (1), pp. 39-47
- Kishida, H., Unzai, Satoru, Roper, David I., Lloyd, A, Park, S.Y., Tame, Jeremy R. H., 2006. Crystal structure of penicillin binding protein 4 (dacB) from Escherichia coli, both in the native form and covalently linked to various antibiotics. Biochemistry, Vol.45 (No.3), pp. 783-792
- 'Schouten, James A., 'Bagga, Sangeev, 'Lloyd, Adrian J., 'De Pascale, Gianfranco, 'Dowson, Christopher G., 'Roper, David I., 'Bugg, Timothy D. H., 2006. 'Fluorescent reagents for in vitro studies of lipid-linked steps of bacterial peptidoglycan biosynthesis: derivatives of UDPMurNAc-pentapeptide containing d-cysteine at position 4 or 5.. Molecular BioSystems, 2 (10), pp. 484-491
- 'Bugg, Timothy D. H., 'Lloyd, Adrian J., 'Roper, David I., 2006. 'Phospho-MurNAc-pentapeptide translocase (MraY) as a target for antibacterial agents and antibacterial proteins. Infectious Disorders - Drug Targets, 6 (2), pp. 85-106
- 'Besong, Gilbert E., 'Bostock, Julieanne M., 'Stubbings, Will, 'Chopra, Ian, 'Roper, David I., 'Lloyd, Adrian J., 'Fishwick, Colin W. G., 'Johnson, A. Peter, 2005. 'A de novo designed inhibitor of D-Ala-D-Ala ligase from E. coli. Angewandte Chemie (International Edition), 44 (39), pp. 6403-6406
- Dhanoa, Gurneet, Roper, David I., Sagona, Antonia P., 2022. Investigation of the localisation of FtsZ in pathogenic E. coli upon bacteriophage addition in a human cell model as a biomarker for antibacterial agents. Access Microbiology, Microbiology Society
- 'Lockey, Christine, 'Edwards, Richard J., 'Roper, David I., 'Dixon, Ann M., 2020. 'Data for The extracellular domain of two-component system sensor kinase VanS from streptomyces coelicolor binds Vancomycin at a newly identified binding site.. University of Warwick, Department of Chemistry
- Kaner, Rebecca A., Allison, Simon A., Faulkner, Alan D., Simpson, Daniel H., Waterfield, Nicholas R., Phillips, Roger M., Roper, David I., Shepherd, Samantha L., Scott, Peter, 2015. Data for Anticancer metallohelices : nanomolar potency and high selectivity. University of Warwick. Science. Chemistry. Scott Group
- 'Lloyd, Adrian J., 'Huyton, Trevor, 'Turkenburg, Johan, 'Roper, David I., 2004. 'Refinement of Haemophilus influenzae diaminopimelic acid epimerase (DapF) at 1.75 A resolution suggests a mechanism for stereocontrol during catalysis. Acta crystallographica. Section D, Biological crystallography, Wiley, pp. 397-400
Title | Funder | Award start | Award end |
---|---|---|---|
Cell Wall Formation in Rod Shaped Bacteria - BBSRC sLoLa Full Stage - February 2023 | BBSRC | 01 Mar 2024 | 31 Jan 2029 |
Antibiotic Breaker- The outer membrane and peptidoglycan link as a new antibiotic vulnerability | Antibiotic Research UK | 02 Sep 2021 | 01 Mar 2023 |
Collaborative visit to India | Academy of Medical Sciences | 04 Nov 2018 | 01 Mar 2023 |
Maintaining cell wall integrity in Gram-Negative Bacteria (Chris Graham) | UK Research and Innovation | 01 Apr 2020 | 30 Sep 2022 |
MRC UK China Hubs | MRC | 01 Jan 2019 | 31 Mar 2022 |
Mechanistic understanding of cell wall biosynthesis to combat antimicrobial resistance: Cell Wall AMR | MRC | 01 Jul 2015 | 28 Aug 2021 |
19 Alert mid-range Equipment Initiative: equipment to upgrade the 600 NMR console at the NMR facility. | BBSRC | 01 Nov 2020 | 31 Mar 2021 |
Understanding how bacteria divide: a route to new antibiotics? (Mr Souvik Naskar) | Medical and Life Sciences Research Fund | 01 Aug 2019 | 31 Dec 2020 |
BBSRC FTMA Investigating the potential to exploit the mechanism of by which tRNA-dependent amino acid synthetases activate cognate and non-cognate amino acids as a strategy for future antibiotic development (Ricky Cain) | BBSRC | 01 Aug 2018 | 31 Jul 2019 |
Accelerate CHNUK AMR discovery: Establishing and exploiting a joint China/UK platform to enable high throughput fragment based inhibitor discovery | MRC | 01 Jul 2016 | 30 Jun 2019 |
17 Alert bid led by Nottingham: A new multi-wavelength analytical ultracentrifuge for the study of biomeolcular interactions | BBSRC | 01 May 2018 | 30 Apr 2019 |
5th Midlands Molecular Microbiology Meeting | Company of Biologists, The | 03 Sep 2018 | 02 Oct 2018 |
Multi-Targetting of tRNA synthetases: A paradigm shift in combating AMR | MRC | 01 Mar 2015 | 30 Nov 2017 |
Molecular Dissection of PASTA domains from Enterococci (Researcher Mobility Grant for Christopher Thoroughgood) | Royal Society of Chemistry | 09 Mar 2017 | 08 Jun 2017 |
Centre for integrative theoretical and experimental analytical science for application to bacterial cell division and Quality by Design (CAS-IDP) | European Commission | 01 Mar 2013 | 28 Feb 2017 |
Team CanUK: Novel antibacterial targets, assays, probes and opportunities in bacterial cell wall biogenesis | MRC | 01 Jan 2012 | 31 Dec 2015 |
BBSRC Link with Astra Zeneca:Reagents and assays to exploit the final steps of peptidoglycan construction | BBSRC | 01 Aug 2013 | 31 Jul 2015 |
The biochemical characterisation of pivotal enzymes involved in mycobacterial peptidoglycan biosynthesis Birmingham-Warwick PG grant | MRC | 01 May 2011 | 30 Apr 2014 |
(led by UCL) BBSRC project Disruption of cytoplasmic membrane-associated functions in staphylococcus aureus by epicatechin gallate led by School of Pharmacy (with Sheffield and Birmingham) | BBSRC | 04 Apr 2011 | 03 Apr 2014 |
Subproject for Institution # 33323 - RCHAR3009 - A new Raman instrument for polarized spectroscopy of biomacromolecular systems | EPSRC | 01 Sep 2012 | 28 Feb 2014 |
A new Raman instrument for polarized spectroscopy of biomacromolecular systems | EPSRC | 01 Sep 2012 | 28 Feb 2014 |
Research Agreement :Investigation into bacterial metabolism - the biochemistry of cytoplasmic synthesis of peptidoglycan precursors entiteld Impact of ligase/MreB association on inhibitor discovery. | Basilea Pharmaceutica International Ltd | 01 Aug 2013 | 31 Jan 2014 |
Unesco- loreal Visiting Fellowship for Vita Majce | UNESCO | 01 May 2012 | 30 Apr 2013 |
UKCan - BACWAN | MRC | 01 Aug 2009 | 30 Apr 2010 |
UK BACWAN 2 | University of Sheffield | 01 May 2008 | 30 Apr 2010 |
Structure and Biology of Membrane Proteins | Wellcome Trust | 01 Jan 2005 | 31 Dec 2009 |
MAR Imaging plate x ray data collection system | Wellcome Trust | 01 Sep 2003 | 31 Aug 2008 |
UK - BaCWAN: UK-Bacterial Cell Wall Assembly Network | MRC | 01 Apr 2006 | 31 Mar 2008 |
Exploration and Exploitation of the Enzymology of PBP Driven Peptidoglycan Biosynthesis to Discover Novel Non-beta Lactam Antibiotics | MRC | 01 Mar 2005 | 29 Feb 2008 |
Towards a complete structure-function description of an aromatic degradative pathway | BBSRC | 01 Feb 2005 | 31 Jan 2008 |
Molecular Physiology and structural analysis of the innate immune system triggered by bacterial cell wall components | British Pharmacological Society | 01 Oct 2004 | 30 Sep 2007 |
Structure, Stability & Molecular Interactions In Biological Systems | BBSRC | 01 Sep 2005 | 31 May 2006 |
Summer projects:I can take on undergraduate students for internships through URSS or other funded schemes (eg Biochem Soc, Welcome Trust) please inquire early!
Final Year UG projects. I take up to 4 laboratory based final year students each year in their 6-7 week undergraduate project. These projects normally encompass PCR amplification, cloning, protein expression, purification and functional testing of a proteins to teach a range of skills within a constrained time period.
I also offer non-lab based final year projects in some aspect of bioscience related to AMR or antibiotic drug discovery.
Masters: I regularly take Mbio 4th year students registered at Warwick.
PhD: I take mini project and PhD students from the MRC DTP and MIBTP programes predominantly but I am open to discussion from other funding sources for studentships including agencies and charities that support overseas nationals. Projects encompass the range of subject areas with the Research Interest descriptions above, please contact me for further info and discussion.
I am happy to receive inquires and discuss PG Research projects in my group, please contact me at the above email address.
In all cases you will be working in an exciting interdisciplinary environment where team science is a guiding principle. We offer sporting transferable skills sessions as well.....