Skip to main content

Dissemination


Summer School on Integrated Technologies for Synthetic Biology and Directed Evolution

Summer School in the University of Warwick co-organized by PLASWIRES and EVOPROG consortia from FET Proactive: Evolving Living Technologies.

The Summer School took place at the University of Warwick and was co-organized by the EVOPROG and PLASWIRES consortia from FET Proactive: Evolving Living Technologies. During the 1st-3rd of July 2015 the participants attended a set of lectures and tutorials aimed at increasing their knowledge on modelling, genetic circuits and customised labware for Synthetic Biology and Directed Evolution experiments.

The aim of this Summer School was to deliver a set of lectures and tutorials that will endow the participants with sufficient knowledge to develop experimental designs integrating modelling, genetic circuits and customised labware for Synthetic Biology and Directed Evolution.

A wide range of backgrounds was present, including synthetic and systems biology, computer science, biomedical and electrical engineering and physics. The make up of talks and tutorials reflected this diversity and promoted active participation of all attendees.

The Summer School presented to the wider community the partners, developments and vision of the EVOPROG and PLASWIRES consortia, with the prospect of generating an open discussion and develop collaborations on the core subjects presented.


Speakers of the SUMMER SCHOOL ON INTEGRATED TECHNOLOGIES FOR SYNTHETIC BIOLOGY AND DIRECTED EVOLUTION included:

- Victor de Lorenzo (Consejo Superior de Investigaciones Cientificas, Spain):TBC

- Alfonso Jaramillo (University of Warwick, UK)

- Mark Isalan (Imperial College London, UK)

- Alfonso Rodríguez-Patón (Universidad Politécnica de Madrid, Spain)

- Vishwesh Kulkarni (University of Warwick, UK)

- Soichiro Tsuda (University of Glasgow, UK)

- Pakpoom Subsoontorn (University of Cambridge, UK)

- Rocio Lopez (Institut Pasteur, France)

- Michal Legiewicz (University of Warwick, UK)

- John Duncan (University of Warwick, UK)

- Andreas Broedel (Imperial College London, UK)

- Manish Kushwaha (University of Warwick, UK)

- Antonia Sagona (University of Warwick, UK)

Programme

The scientific programme focused on the skills existing within the EVOPROG and PLASWIRES consortia and included some speakers from the University of Warwick.

Day 1 - Wednesday
9am – 9.30am Welcome and Introduction to the Summer School
9.30am - 1pm Session 1 (Coffee break 11.00am to 11.15am)
 

"Simulating multicellular genetic circuits with an individual-based model: new features in GRO simulator."

Dr Alfonso Rodríguez-Patón (Universidad Politécnica de Madrid, Spain)

 

"Diversity Generating Retroelements"

Dr Michal Legiewicz (University of Warwick, UK)

 

"Synthesizing Programmable Biomolecular Circuits"

Dr Vishwesh Kulkarni (University of Warwick, UK)

1pm – 2pm

Lunch

2pm - 5.30pm

Session 2 (Coffee break 3.30pm to 3.45pm)

  "Computational modeling of multicellular bacterial populations"

Dr Alfonso Rodríguez-Patón (Universidad Politécnica de Madrid, Spain)

Dr John Duncan (University of Warwick, UK)

Mr Pakpoom Subsoontorn (University of Cambridge, UK)

Mr Martin Gutierrez (Universidad Politécnica de Madrid, Spain)

Day 2 - Thursday
9am - 1pm Session 3 (Coffee break 11.00am to 11.15am)
 

"New tools for gene evolution in vivo"

Dr Yamal González (Consejo Superior de Investigaciones Cientificas, Spain)

 

"RNA technologies to engineer gene circuits in bacteria"

Prof Alfonso Jaramillo (University of Warwick, UK)

 

"Engineering synthetic patterns inspired by development"

Dr Mark Isalan (Imperial College London, UK)

1pm – 2pm Lunch
2pm - 5.30pm Session 4 (Coffee break 3.30pm to 3.45pm)
 

"Phage production and analysis of the titer"

Dr Andreas Broedel (Imperial College London, UK)

Dr Yamal González (Consejo Superior de Investigaciones Cientificas, Spain)

Dr Antonia Sagona (University of Warwick, UK)

Day 3 - Friday
9am - 10.45am Session 5
 

"Synthetic biology in Cyanobacteria"

Dr Rocio Lopez (Institut Pasteur, France)

 

"Designing Portable Gene Expression for Cross-species Application"

Dr Manish Kushwaha (University of Warwick, UK)

10.45am - 11am Coffee break
11am - 3.30pm Session 6 (Lunch 1pm to 2pm)
  "Creation of reproducible custom components for the lab"
  Dr Rui Rodrigues (University of Warwick, UK)
  Dr Soichiro Tsuda (University of Glasgow, UK)
  Mr Ismael Garcia (Universidad Politécnica de Madrid, Spain)
3.30pm – 4pm Final notes on the Summer School


Event Summary

A Summer School with the objective of increasing cooperation and expertise exchange between FET projects EVOPROG and PLASWIRES was organized and held at the University of Warwick from the 1st to the 3rd of July 2015.

The Summer School on Integrated Technologies for Synthetic Biology and Directed Evolution and was attended by 32 participants of different backgrounds (mathematics, biology, bioengineering, synthetic biology and physics), including students attending different programs at the University of Warwick.

The school covered a range of topics of broad interest relating to modern world such as energy, novel biologicals for healthcare and biotechnology or emerging innovative technologies for life sciences and bioengineering. Tutors and lecturers shared theirs expertise in protein engineering, 3D printing, microbiology, synthetic biology, directed evolution, and genome engineering.

Each day was divided into morning and afternoon sessions. The morning sessions involved talks on applications of riboregulators for synthetic biology, engineering programmable genetic circuits to control gene expression, exploitation of cyanobacteria for practical applications, strategies for controlling gene diversification for directed evolution, and advancements in genome engineering.

The afternoon sessions were laboratory-based activities and provided opportunity for gaining hands-on experience in operations on bacterial cells, modelling in 2D and 3D spaces, phage production and titration as an introduction for phage assisted continuous evolution and also robust phage display for antibody development, 3D printing and integration of 3D objects with sensors and sensing/control using Arduino boards.

The participants gained transferable sets of skills valuable for both academia and industry, which will be beneficial for their future careers. The techniques taught are particularly relevant for those looking for future employment in facilities operating with bacterial cultures (fermentation), phages (evolution of biomolecules, antibody engineering), 3D designing and printing (hardware engineering), or industrial setups operating on modified organisms for commercial bioproduct formation. Each day was concluded with a social event held within the University, which extended the opportunities for professional networking, exchange of ideas and establishing future interdisciplinary collaborations.

Pictures of the event


SummerSchool1SummerSchool2_2

SummerSchool_3SummerSchool4_2

SummerSchool5_2SummerSchool6_2

SummerSchool7_2SummerSchool8_2