Warwick/Waters Centre for BioMedical Mass Spectrometry and Proteomics

The Warwick/Waters Centre for BioMedical Mass Spectrometry and Proteomics in the School of Life Sciences, University of Warwick houses internationally competitive MS-based instrumentation. The Centre undertakes collaborative proteomics-based projects with both U.K. and international academic institutions on a number of both well characterised and novel organisms. Projects include protein profiling, quantitative proteomic studies, phosphorylation and post-translational modification mapping. The use of bioinformatic approaches is crucial to a significant number of the projects undertaken, with four dual processor servers, 20 Tb of data storage capacity and a bioinformatics cluster (Apple) based in the Centre for Systems Biology available.

The Centre houses four ESI Q-ToF instruments, situated in containment laboratories:

  • Synapt G2 HDMS with a nanoAcquity LC system (Waters)
  • Synapt HDMS for travelling wave-based ion mobility separations of gas phase molecules with a dedicated nanoAcquity LC system (Waters)
  • Ultima Global with a dedicated nanoAcquity LC system (Waters)
  • Ultima API-US (Waters)

 

Additional MS instrumentation includes:

  • Xevo TQ with Acquity/NanoAcquity LC systems (Waters)
  • TQD with Acquity LC system (Waters)
  • MALDI micro MX with multiplexed PSD facility (Waters)
  • LTQ-XL based at Wellesbourne (Thermo)
  • GC-MS (Hewlett Packard-Trio)

 

Quantitative proteomic studies include the use of:

  • Protein Expression Profiling System (Waters)
  • Absolute quantitation (AQUA™/QconCAT peptides)
  • Chemical labelling methods including iTRAQ (Applied BioSystems) and SILAC
  • Solution-based protein fractionation (ProteomeLab PF2D, Beckman Coulter)

 

For further details and enquiries please email Susan Slade or telephone 024 7615 0439.