Gifford lab group members
miriam dot gifford at warwick dot ac dot uk
|Synthetic Biology CDT PhD student||P dot Morrison at warwick dot ac dot uk|
EPSRC Systems Biology PhD student
T dot L dot R dot Coker at warwick dot ac dot uk
MIBTP Life Sciences PhD student
|M dot Achom at warwick dot ac dot uk|
MIBTP Life Sciences PhD student
M dot Teft at warwick dot ac dot uk
MIBTP Life Sciences pre-PhD student
Liam dot Walker at warwick dot ac dot uk
We are part of the School of Life Sciences & Warwick Integrative Synthetic Biology.
Understanding then rewiring nodulation:
Unlike most plants, legumes (such as peas and beans) are able to fix atmospheric nitrogen (nodulation), so break the agricultural dependency on expensive and environmentally costly nitrogen fertilisers. If we understand the molecular basis of why legumes nodulate but other plants don't we could improve nitrogen-use efficiency in our crops. This would improve food security, a major research priority and one within the agri-tech strategy remit.
How does cell identity affect nitrogen responses and nodulation, and can we re-tune it?
We use Fluorescence-Activated Cell Sorting (FACS) combined with genomics to measure the gene expression of all regulatory pathways and interpret them in the context of root phenotypes. This is critical since nodules develop from a specific root cell type and root environmental responses have a high degree of cell specificity. We also want to investigate how rhizobial responses (nodulation) intersect with responses to other mutualists in the rhizosphere to improve plant productivity.
Exploiting cross-talk to direct crop improvement:
To create a realistic model of plant resource use it is critical to study the combined effect of many environmental factors since plants have to balance many selective pressures. For example, acquiring a feature that has a positive impact on water uptake might also have a negative impact on disease resistance. Our work in this area includes:
Engineering microbial effector systems in plants:
Application of cell type analysis to delineate genes acting in specific locations and under specific conditions. We can use this to predict which promoters and genes could be engineered to enable new functions, including altered plant disease responses and altered mutualistic responses (Theme 4 in the Warwick Integrative Synthetic Biology Centre).
MScs and MBios:
Will Macnair (Systems Biology MSc project student) - PhD with Prof. Manfred Claassen (University of Zurich)
Waqar Yousaf (MBio project student) - PGCE study and Teacher training
Dr Roxanna Bonyadi (Chancellor's International Scholarship PhD student)
Dr Anthony Carter (EPSRC Systems Biology PhD student) - Statistician in the Civil Service (UK)
Jo Hulsmans (Cypress Diagnostics, Belgium)
Dr Zennia Paniwynk (Defra Post-doc) - Scientist at Quanta Fluid Solutions (UK)
Dr Jesper Grønlund (BBSRC Post-doc) - Gene Discovery Manager at Advanced Technologies Cambridge (BAT, UK)
Dr Sajjad Awan (Defra Post-doc) - Post-doc with Prof. Bill Finch-Savage (Life Sciences, Warwick, UK)
Sanjeev Kumar (BBSRC Research technician) - Technician with Prof. David Twell (Leicester, UK), now at VWR
Dr Sally Adams (Post-doc) - Post-doc with Dr Andre Pires da Silva (Life Sciences, Warwick, UK)
Dr Ying Wang (BBSRC Post-doc) - Post-doc (Germany)
Dr. Dhaval Patel (BBSRC Post-doc) - Scientist at Azotic Technologies Ltd (Nottingham, UK)
Dr. Dafyd Jenkins (BBSRC Post-doc) - Data scientist at TATA (Coventry, UK)
Latest info on our research:
Launch of WISB: plant synthetic biology research including insights gained from cell type analysis.
New paper in Plant Cell - collaboration with Oldroyd lab on tissue specific coordination of nodulation by NIN transcription factor
Collaborative PhD studentship with Lionel Dupuy & Nicola Holden at James Hutton: Growing Rhizoplanes
Our New Phytologist Cell type specific proteomics workshop:
WATCH our JOVE cell sorting video!
Arabidopsis ready for sorting: