Laboratory Skills Course
Practical Laboratory Training in Molecular Biology & Protein Expression
Course Introduction
The vast majority of this two week intensive course consists of hands-on laboratory work, providing the opportunity to learn vital and fundamental laboratory skills. An excellent theoretical background to the techniques is provided through accompanying discussion. The course will provide the opportunity to demonstrate your skill in the laboratory to employers through your own individual lab book write-up. The course will also give an insight into state-of-the-art methodologies used in a broad range of topics.
Who should attend
This course is deisgned for any one wanting a basic, hands-on introduction to practical biochemistry and molecular biology techniques including new graduates, health professionals and pharmaceutical industry personnel.
It is particularly suitable for Biotechnology, Bioprocessing and Business Management MSc students who need laboratory experience.
Delegates can choose to attend either week or the whole course.
Course Content
Week1
Fundamentals of lab work. To include;
- Welcome and Introduction
- Safety
- Weights and measures
- What is a buffer? Calibration and use of a pH meter
- Transformation of E.coli with plasmid DNA
Purification and analysis of plasmid DNA. To include;
- Mini-preps
- Agarose gel electrophoresis
- Restriction digests
- Making plates
PCR mutagenesis techniques. To include;
- Preparation of DNA for sequencing
- Polymerase Chain Reaction
- Primer design
Cloning into an E.coli expression vector. To include;
- sequencing
- analysis of sequencing data by computer
Site directed mutagenesis
Light and Electron Microscopy (both weeks)
Week 2
Overexpression of protein in E.coli. To include;
- Growing a culture
- Making a buffer
- Lactate dehydrogenase (LDH) expression
Purification of protein using affinity chromatography and SDS gel electrophoresis. To include;
- Use of sonicator or chemical cell lysis
- Running a column
- SDS gel electrophoresis
Protein analysis. To include;
- SDS gel interpretation
- spectrophotometric enzyme assays
- kinetic analysis
Western blots
Experimental design
Dates of next course:
3rd - 14th September 2012
Price:
£999 for whole course
£600 for a single week.
Includes all lab materials and course manual.
E mail:
Charlotte.Moonan@warwick.ac.uk
Leaflet & Application Form:
