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TM data

Sheet1

DNA

 

DNA+Fe2L3

 

Melting curves in 10 mM NaCl and 1 mM sod. cacod.

t

Abs

t

Abs

 

DNA:Fe2L3 10:1

20.01

0.9999996

20.02

1

   

20.5

1.000004

20.56

0.9996928

   

21

0.9999106

21.06

0.9997333

   

21.5

0.9994969

21.54

0.9997312

   

22

0.9999102

22.05

0.9997545

   

22.51

1.000054

22.54

0.999759

   

23

1.000098

23.05

0.9997571

   

23.5

1.000026

23.56

0.9997513

   

24.01

0.9998956

24.06

1.00003

   

24.5

0.9997882

24.54

0.9994319

   

25

0.9999

25.04

0.9997324

   

25.51

0.9997175

25.56

0.9997304

   

26

0.9996541

26.03

0.9997288

   

26.51

0.9999139

26.55

0.9997183

   

27

0.9995358

27.06

0.9994183

   

27.5

0.9994928

27.54

0.9997816

   

28.01

0.9986202

28.06

1

   

28.5

0.9986484

28.55

0.9996928

   

29

0.9986394

29.04

0.9997333

   

29.5

0.9985799

29.55

0.9997312

   

30.01

0.9985648

30.05

0.9997545

   

30.5

0.998608

30.55

0.999759

   

31

0.9986449

31.04

0.9997571

   

31.5

0.9986277

31.54

0.9997513

   

32

0.998648

32.03

1.001084

   

32.49

0.9986966

32.56

1.001024

   

32.99

0.9986941

33.04

0.9997324

   

33.5

0.9987001

33.53

0.9997304

   

33.99

0.9972383

34.06

0.9997288

   

34.5

0.997252

34.54

0.9997183

   

34.99

0.9972357

35.04

0.9998024

   

35.5

0.9972121

35.54

0.9998062

   

36.01

0.9972423

36.03

0.9997955

   

36.49

0.99727

36.55

0.9997931

   

37

0.9972694

37.03

0.9998123

   

37.49

0.9972277

37.54

0.9998028

   

37.99

0.9972293

38.04

0.9998047

   

38.5

0.9972293

38.53

0.9998096

   

38.99

0.9972551

39.05

0.9997813

   

39.5

0.9972071

39.54

0.9997848

   

39.99

0.9972059

40.04

0.9997827

   

40.49

0.9972008

40.54

0.9997655

   

41

0.997204

41.03

0.9997752

   

41.5

0.9972

41.54

0.9997886

   

42

0.9972166

42.05

0.9997813

   

42.5

0.9972061

42.52

0.9997621

   

43

0.9972222

43.04

0.9997521

   

43.5

0.9972104

43.56

0.9997621

   

43.99

0.9967001

44.03

0.9997577

   

44.5

0.9970288

44.55

0.99977

   

44.99

0.9961742

45.02

0.9997624

   

45.49

0.9960612

45.54

0.9997486

   

46

0.9967779

46.04

0.9990879

   

46.5

0.9952921

46.54

0.9990478

   

47

0.9963658

47.04

0.9990829

   

47.49

0.9963443

47.55

0.9991047

   

47.99

0.9953181

48.05

0.9990505

   

48.5

0.9964243

48.54

0.9988356

   

49

0.9960676

49.03

0.9989048

   

49.5

0.9949332

49.54

0.9987792

   

50

0.9953164

50.04

0.9986833

   

50.49

0.9954103

50.53

0.9990207

   

50.99

0.9950936

51.05

0.9987361

   

51.5

0.9951104

51.54

0.9988883

   

51.99

0.99467

52.04

0.9990551

   

52.5

0.9945261

52.56

0.9989389

   

53

0.9943638

53.06

0.9989106

   

53.49

0.9940106

53.54

0.9990246

   

54

0.9937617

54.02

0.9987494

   

54.5

0.993961

54.54

0.9988099

   

55

0.9935622

55.04

0.9985712

   

55.5

0.9936892

55.56

0.9985852

   

55.99

0.9933214

56.04

0.9985189

   

56.5

0.9936264

56.54

0.9983789

   

56.99

0.9933057

57.06

0.9983632

   

57.49

0.9942311

57.54

0.9982402

   

58.01

0.9944937

58.04

0.9981536

   

58.5

0.994696

58.55

0.9980252

   

58.99

0.9960797

59.03

0.9980925

   

59.5

0.9968229

59.54

0.9979373

   

60

0.9989562

60.04

0.997836

   

60.5

1.001514

60.55

0.9976929

   

61

1.00526

61.06

0.9976409

   

61.5

1.010095

61.54

0.9984636

   

62

1.016456

62.03

0.9981809

   

62.49

1.023842

62.54

0.9989688

   

62.99

1.032167

63.06

0.9987051

   

63.5

1.041402

63.54

0.9984803

   

64

1.050492

64.06

0.9976122

   

64.5

1.05959

64.55

0.9969458

   

65

1.070124

65.07

0.9958135

   

65.51

1.080212

65.54

0.9945064

   

66

1.090554

66.05

0.9953344

   

66.51

1.101817

66.55

0.9967188

   

67

1.113211

67.05

0.9968058

   

67.5

1.123822

67.54

0.9980826

   

68

1.13345

68.06

0.9989784

   

68.5

1.143018

68.55

1.003228

   

69

1.151395

69.05

1.00718

   

69.5

1.15875

69.56

1.012539

   

70.01

1.165885

70.05

1.019731

   

70.51

1.172257

70.56

1.026499

   

71.01

1.178556

71.06

1.034986

   

71.5

1.185341

71.52

1.043099

   

72

1.192372

72.03

1.05224

   

72.5

1.197937

72.55

1.063246

   

73

1.201774

73.06

1.073105

   

73.5

1.204958

73.54

1.082173

   

74.01

1.2079

74.05

1.09267

   

74.5

1.211636

74.56

1.102552

   

75.02

1.216137

75.05

1.112242

   

75.5

1.220099

75.55

1.12009

   

76

1.224339

76.06

1.12721

   

76.5

1.227497

76.54

1.136176

   

77

1.229456

77.05

1.145406

   

77.52

1.230443

77.55

1.152498

   

78

1.231328

78.04

1.160493

   

78.5

1.233052

78.54

1.1674

   

79

1.234564

79.04

1.173436

   

79.5

1.236214

79.56

1.178892

   

80

1.238064

80.04

1.185298

   

80.5

1.239728

80.55

1.190727

   

81.01

1.241068

81.05

1.194909

   

81.51

1.242304

81.54

1.199369

   

82.01

1.243272

82.04

1.203449

   

82.5

1.244042

82.56

1.206263

   

83

1.244898

83.07

1.210174

   

83.5

1.245832

83.55

1.213446

   

84.02

1.24652

84.04

1.21567

   

84.51

1.246483

84.55

1.217792

   

85.01

1.247066

85.05

1.219938

   

85.51

1.247132

85.55

1.22312

   

86.02

1.247199

86.06

1.225445

   

86.5

1.247755

86.54

1.226686

   

87

1.247798

87.04

1.229053

   

87.52

1.247629

87.54

1.231292

   

88

1.247711

88.06

1.233143

   

88.5

1.248172

88.56

1.234811

   

89

1.24834

89.04

1.23565

   

89.5

1.248286

89.55

1.237188

   

90.01

1.248465

90.05

1.239808

   

90.5

1.2484

90.55

1.241093

   

91

1.24847

91.06

1.242692

   

91.51

1.248645

91.55

1.244178

   

92

1.248948

92.06

1.244951

   

92.5

1.249278

92.56

1.246133

   

93

1.249342

93.06

1.248828

   

93.51

1.249532

93.55

1.250618

   

94

1.249421

94.04

1.251419

   

94.51

1.249557

94.55

1.252342

   

95

1.249776

95.04

1.254328