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Claire Broughton

I studied Biochemistry at King's College London graduating with first class honours in 2007.

My BSc research project investigated changes to gene expression profiles in skin cells on exposure to agents such as nicotine and UV radiation. This work was nominated for the National Undergraduate Science, Engineering and Technology Award 2007.

I spent three years working in IT Project Management and Credit Risk for Barclays Bank before joining MOAC in 2010.

I completed an MSc in Mathematical Biology and Biophysical Chemistry with the MOAC DTC and graduated with Distinction in 2011.

I then completed my PhD in Mathematical Biology and Biophysical Chemistry in 2016. My interdisciplinary project was titled A Predictive Model of Bacterial Cell Division and was co-supervised by Dr Hugo van den Berg, Professor Alison Rodger and Dr David Roper.

MOAC MSc 2010-2011 - Mathematical Biology and Biophysical Chemistry


Mini-Project 1

My first mini-project was supervised by Dr Victoria Marlow and Professor Vilmos Fulop in the Structural Biology Group of the School of Life Sciences.

Towards determining the catalytic mechanism of the enzyme acetolactate decarboxylase (ALDC), I used site-directed mutagenesis to produce variants of the enzyme, each with a single residue change in the active site. The mutant proteins were expressed in E. coli and purified by chromatography. I also used circular dichroism to confirm that the secondary structure of the protein had been retained, despite the mutation, and then began initial assays to characterise activity. More information

Mini-Project 2

My second mini-project was supervised by Professor Andrew Dove in the Chemistry Department. My poster was awarded runner-up in the MSc poster prize at the MOAC DTC Annual Conference 2011.

To develop degradable plastics for tissue engineering scaffolds, I synthesised a ketene acetal monomer for blending into UV-cured materials. The current materials partially degrade but long polyacrylate chains remain which may be difficult for the body to clear during tissue regrowth. Addition of up to 20% of the synthesised ketene acetal to the polymer maintained the tensile strength of the original material so further degradation studies will confirm if the new blends give the required degradation rates. More information

Mini-Project 3

My third mini-project was supervised by Dr Hugo van den Berg of the Mathematics Institute.

This work forms part of a larger collaborative effort across various research groups at Warwick to investigate the cell division machinery of prokaryotic cells. The aim of my project was to develop a mathematical model of the formation and maintenance of the Z-ring in E. coli. This work was continued in my PhD project.

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MOAC DTC
Coventry House
University of Warwick
Coventry
CV4 7AL

c dot broughton at warwick dot ac dot uk